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VisEn Medical prosense680™
Prosense680™, supplied by VisEn Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prosense680/prosense680/us10730983-492-23-25
Average 90 stars, based on 1 article reviews
prosense680™ - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Imaging:

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. Panel A: Visen FMT imaging of tumor volume at 12 days post visible tumor formation in wildtype C57BL/6 mouse (Left Panel) or MOR knockout mouse (Right Panel) after injecting Prosense680 (i.v, 2nM, 24 hours prior imaging) near infrared probe for quantification of tumor volume and lung metastasis. ..

Article Title: Detection of oral squamous cell carcinoma and cervical lymph node metastasis using activatable near-infrared fluorescence agents.
Article Snippet: .. METHODS REAGENTS The NIRF agent ProSense680 (VisEn Medical, Woburn, Massachusetts), with peak excitation at 680 nm and emission at 700 nm, was used for imaging of the tumor and involved margins. .. ProSense680 is conjugated to the fluorochrome VivoTagS680 (VisEn Medical) and becomes activated after cleavage by proteolytic activation of lysosomal cysteine or serine proteases, including cathepsin B.

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. To test this, LLC cells were injected into the hind flank of wildtype and MOR knockout mice and tumor formation was examined in vivo using Visen FMT imaging of tumor volume at 12 days post visible tumor formation after injection of Prosense680 33 , 34 (activated by cathepsins, known to be up-regulated in cancer 35 ) near infrared probe ( ). ..

Knock-Out:

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. Panel A: Visen FMT imaging of tumor volume at 12 days post visible tumor formation in wildtype C57BL/6 mouse (Left Panel) or MOR knockout mouse (Right Panel) after injecting Prosense680 (i.v, 2nM, 24 hours prior imaging) near infrared probe for quantification of tumor volume and lung metastasis. ..

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. To test this, LLC cells were injected into the hind flank of wildtype and MOR knockout mice and tumor formation was examined in vivo using Visen FMT imaging of tumor volume at 12 days post visible tumor formation after injection of Prosense680 33 , 34 (activated by cathepsins, known to be up-regulated in cancer 35 ) near infrared probe ( ). ..

Generated:

Article Title: Imaging systems featuring waveguiding compensation
Article Snippet: .. Scale factors can be generated to account for the photochemical properties of different fluorescent agents, such as ProSense680, OsteoSense750 and others (VisEn Medical, Woburn, Mass.). .. This calibration process may be repeated several times per agent to increase the statistical robustness of the calibration and decrease the impact of operator variability, batch to batch agent variability, and other sources of error.

Article Title: Functional in vivo imaging of cysteine cathepsin activity in murine model of inflammation.
Article Snippet: Near-infrared fluorophore (NIRF)-labeled imaging probes are becoming increasingly important in bio-molecular imaging applications, that is, in animal models for tumor imaging or inflammation studies.. In this study we showed that the previously introduced chemical concept of ‘Reverse Design’ represents an efficient strategy for the generation of selective probes for cysteine proteases from chemically optimized protease inhibitors for investigations in proteomic lysates as well as for in vivo molecular imaging studies.. The newly developed activity-based probe AW-091 was demonstrated to be highly selective for cathepsin S in vitro and proved useful in monitoring cysteine cathepsin activity in vivo, that is, in zymosan-induced mouse model of inflammation.

Fluorescence:

Article Title: Image-guided tumor resection using real-time near-infrared fluorescence in a syngeneic rat model of primary breast cancer.
Article Snippet: Tumor involvement of resection margins is found in a large proportion of patients who undergo breastconserving surgery.. Near-infrared (NIR) fluorescence imaging is an experimental technique to visualize cancer cells during surgery.. To determine the accuracy of realtime NIR fluorescence imaging in obtaining tumor-free resection margins, a protease-activatable NIR fluorescence probe and an intraoperative camera system were used in the EMR86 orthotopic syngeneic breast cancer rat model.

Activation Assay:

Article Title: Detection of oral squamous cell carcinoma and cervical lymph node metastasis using activatable near-infrared fluorescence agents.
Article Snippet: METHODS REAGENTS The NIRF agent ProSense680 (VisEn Medical, Woburn, Massachusetts), with peak excitation at 680 nm and emission at 700 nm, was used for imaging of the tumor and involved margins. .. ProSense680 is conjugated to the fluorochrome VivoTagS680 (VisEn Medical) and becomes activated after cleavage by proteolytic activation of lysosomal cysteine or serine proteases, including cathepsin B. .. The second agent used was MMPSense680 (VisEn Medical), which is conjugated to the same fluorochrome and has the same fluorescence characteristics.

Injection:

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. To test this, LLC cells were injected into the hind flank of wildtype and MOR knockout mice and tumor formation was examined in vivo using Visen FMT imaging of tumor volume at 12 days post visible tumor formation after injection of Prosense680 33 , 34 (activated by cathepsins, known to be up-regulated in cancer 35 ) near infrared probe ( ). ..

In Vivo:

Article Title: Novel Role of the Mu Opioid Receptor in Lung Cancer Progression: A Laboratory Study
Article Snippet: .. To test this, LLC cells were injected into the hind flank of wildtype and MOR knockout mice and tumor formation was examined in vivo using Visen FMT imaging of tumor volume at 12 days post visible tumor formation after injection of Prosense680 33 , 34 (activated by cathepsins, known to be up-regulated in cancer 35 ) near infrared probe ( ). ..

Polymer:

Article Title: Functional in vivo imaging of cysteine cathepsin activity in murine model of inflammation.
Article Snippet: Near-infrared fluorophore (NIRF)-labeled imaging probes are becoming increasingly important in bio-molecular imaging applications, that is, in animal models for tumor imaging or inflammation studies.. In this study we showed that the previously introduced chemical concept of ‘Reverse Design’ represents an efficient strategy for the generation of selective probes for cysteine proteases from chemically optimized protease inhibitors for investigations in proteomic lysates as well as for in vivo molecular imaging studies.. The newly developed activity-based probe AW-091 was demonstrated to be highly selective for cathepsin S in vitro and proved useful in monitoring cysteine cathepsin activity in vivo, that is, in zymosan-induced mouse model of inflammation.



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ProSense Inc biomarker prosense680
a Schematic of analysis pipeline overlaying brain-region-defining genes identified with bulk-sequencing onto mpeg1.1 + scRNA-sequencing clusters. Colors correspond to clusters defined in Fig. . b Feature plots of region-defining eigengenes from hindbrain (HB; red), midbrain (MB; green), and optic tectum (OT; blue) overlaid on mpeg1.1 + UMAP, highlighting microglial clusters JM1 and JM4. Eigengenes were composed of single region marker genes with regional enrichment (log fold change >1.2, basemean >100, and adj. p < 0.05) from the bulk-sequencing analysis in Fig. and computed with the PercentageFeatureSet function in the Seurat R package. Color-coding for Eigengene expression: OT, low expression; light blue, high expression; dark blue. MB, low expression; light green, high expression; dark green. HB, low expression; pink, high expression; red. c Violin plots of regional eigengene distributions, related to b . Colors correspond with clusters shown in Figs. c, 4a. (Solid black line = median; dotted lines = 1st and 3rd quartiles). d Volcano plot of differentially expressed genes between clusters JM1 (OT-enriched; blue) and JM4 (hindbrain-enriched; red). Thresholds represented by dotted lines were set to adjusted p value < 10 −8 , log fold change >0.2. (MAST differential expression test with Bonferroni correction; See Supplemental S5). e Top GO terms from differentially expressed genes in d . (Metascape; hypergeometric test with Benjamini–Hochberg correction for multiple comparisons). f Representative images of cathepsin-cleaved <t>Prosense680</t> colocalized with mpeg1.1- EGFP in indicated brain regions. Scale: 100 µm. Images are representative of the n = 3 replicates. g Quantification of total percent microglia containing cleaved Prosense680 in OT and HB. Unpaired t tests. Dots represent three individual fish, data are mean ± SD. * p < 0.0236. OT (Optic Tectum) and HB (Hindbrain). Two-tailed unpaired t test. h Representative images and 3D reconstructions of mpeg1.1- EGFP+ microglia with engulfed SV2 protein. Insets: close-up of reconstructions (arrowheads:SV2 content). Scale bar 5 µm. Images are representative of ameboid or ramified morphology. i Quantification of percent microglial volume containing SV2 in randomly selected microglia from OT and HB. Post hoc analyses show morphology assignment as ramified (red, sphericity <0.6) vs. ameboid (blue, sphericity >0.6. Mann–Whitney U test, * p < 0.0177, dots = mean value per fish from three microglia per fish. See also Fig. and Fig. , Supplementary Data .
Biomarker Prosense680, supplied by ProSense Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prosense680/prosense+680/pmc08501082-172-17-43
Average 90 stars, based on 1 article reviews
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VisEn Medical prosense680™
a Schematic of analysis pipeline overlaying brain-region-defining genes identified with bulk-sequencing onto mpeg1.1 + scRNA-sequencing clusters. Colors correspond to clusters defined in Fig. . b Feature plots of region-defining eigengenes from hindbrain (HB; red), midbrain (MB; green), and optic tectum (OT; blue) overlaid on mpeg1.1 + UMAP, highlighting microglial clusters JM1 and JM4. Eigengenes were composed of single region marker genes with regional enrichment (log fold change >1.2, basemean >100, and adj. p < 0.05) from the bulk-sequencing analysis in Fig. and computed with the PercentageFeatureSet function in the Seurat R package. Color-coding for Eigengene expression: OT, low expression; light blue, high expression; dark blue. MB, low expression; light green, high expression; dark green. HB, low expression; pink, high expression; red. c Violin plots of regional eigengene distributions, related to b . Colors correspond with clusters shown in Figs. c, 4a. (Solid black line = median; dotted lines = 1st and 3rd quartiles). d Volcano plot of differentially expressed genes between clusters JM1 (OT-enriched; blue) and JM4 (hindbrain-enriched; red). Thresholds represented by dotted lines were set to adjusted p value < 10 −8 , log fold change >0.2. (MAST differential expression test with Bonferroni correction; See Supplemental S5). e Top GO terms from differentially expressed genes in d . (Metascape; hypergeometric test with Benjamini–Hochberg correction for multiple comparisons). f Representative images of cathepsin-cleaved <t>Prosense680</t> colocalized with mpeg1.1- EGFP in indicated brain regions. Scale: 100 µm. Images are representative of the n = 3 replicates. g Quantification of total percent microglia containing cleaved Prosense680 in OT and HB. Unpaired t tests. Dots represent three individual fish, data are mean ± SD. * p < 0.0236. OT (Optic Tectum) and HB (Hindbrain). Two-tailed unpaired t test. h Representative images and 3D reconstructions of mpeg1.1- EGFP+ microglia with engulfed SV2 protein. Insets: close-up of reconstructions (arrowheads:SV2 content). Scale bar 5 µm. Images are representative of ameboid or ramified morphology. i Quantification of percent microglial volume containing SV2 in randomly selected microglia from OT and HB. Post hoc analyses show morphology assignment as ramified (red, sphericity <0.6) vs. ameboid (blue, sphericity >0.6. Mann–Whitney U test, * p < 0.0177, dots = mean value per fish from three microglia per fish. See also Fig. and Fig. , Supplementary Data .
Prosense680™, supplied by VisEn Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prosense680/prosense680/us10730983-492-23-25
Average 90 stars, based on 1 article reviews
prosense680™ - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
VisEn Medical prosense680
a Schematic of analysis pipeline overlaying brain-region-defining genes identified with bulk-sequencing onto mpeg1.1 + scRNA-sequencing clusters. Colors correspond to clusters defined in Fig. . b Feature plots of region-defining eigengenes from hindbrain (HB; red), midbrain (MB; green), and optic tectum (OT; blue) overlaid on mpeg1.1 + UMAP, highlighting microglial clusters JM1 and JM4. Eigengenes were composed of single region marker genes with regional enrichment (log fold change >1.2, basemean >100, and adj. p < 0.05) from the bulk-sequencing analysis in Fig. and computed with the PercentageFeatureSet function in the Seurat R package. Color-coding for Eigengene expression: OT, low expression; light blue, high expression; dark blue. MB, low expression; light green, high expression; dark green. HB, low expression; pink, high expression; red. c Violin plots of regional eigengene distributions, related to b . Colors correspond with clusters shown in Figs. c, 4a. (Solid black line = median; dotted lines = 1st and 3rd quartiles). d Volcano plot of differentially expressed genes between clusters JM1 (OT-enriched; blue) and JM4 (hindbrain-enriched; red). Thresholds represented by dotted lines were set to adjusted p value < 10 −8 , log fold change >0.2. (MAST differential expression test with Bonferroni correction; See Supplemental S5). e Top GO terms from differentially expressed genes in d . (Metascape; hypergeometric test with Benjamini–Hochberg correction for multiple comparisons). f Representative images of cathepsin-cleaved <t>Prosense680</t> colocalized with mpeg1.1- EGFP in indicated brain regions. Scale: 100 µm. Images are representative of the n = 3 replicates. g Quantification of total percent microglia containing cleaved Prosense680 in OT and HB. Unpaired t tests. Dots represent three individual fish, data are mean ± SD. * p < 0.0236. OT (Optic Tectum) and HB (Hindbrain). Two-tailed unpaired t test. h Representative images and 3D reconstructions of mpeg1.1- EGFP+ microglia with engulfed SV2 protein. Insets: close-up of reconstructions (arrowheads:SV2 content). Scale bar 5 µm. Images are representative of ameboid or ramified morphology. i Quantification of percent microglial volume containing SV2 in randomly selected microglia from OT and HB. Post hoc analyses show morphology assignment as ramified (red, sphericity <0.6) vs. ameboid (blue, sphericity >0.6. Mann–Whitney U test, * p < 0.0177, dots = mean value per fish from three microglia per fish. See also Fig. and Fig. , Supplementary Data .
Prosense680, supplied by VisEn Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prosense680/prosense680/us09311722-357-17-21
Average 90 stars, based on 1 article reviews
prosense680 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Table of Materials

Journal: Journal of visualized experiments : JoVE

Article Title: Non-Invasive Compression-Induced ACL Injury and In Vivo Imaging of Protease Activity in Mice

doi: 10.3791/65249

Figure Lengend Snippet: Table of Materials

Article Snippet: Name of Material/ Equipment Company Catalog Number Comments/Description Materials testing systems TA Instruments Electroforce 3200 or equivalent Uniaxial load cell TA Instruments 20 N capacity Fixtures Custom-made knee fixture, ankle fixture, and platform Air Anesthetia System Isoflurane vaporizor with induction chamber and nose cone Buprenorphine Analgesic post-injury IVIS Spectrum Perkin Elmer 124262 Can also use comparable optical imaging system ProSense680 Perkin Elmer NEV10003 Can also use other probes such as OsteoSense, MMPSense, Cat K, AngioSense, etc.

Techniques: Optical Imaging, Cream, Saline, Sterility

Table of Materials

Journal: Journal of visualized experiments : JoVE

Article Title: Non-Invasive Compression-Induced ACL Injury and In Vivo Imaging of Protease Activity in Mice

doi: 10.3791/65249

Figure Lengend Snippet: Table of Materials

Article Snippet: ProSense680 , Perkin Elmer , NEV10003 , Can also use other probes such as OsteoSense, MMPSense, Cat K, AngioSense, etc..

Techniques: Optical Imaging, Cream, Saline, Sterility

a Schematic of analysis pipeline overlaying brain-region-defining genes identified with bulk-sequencing onto mpeg1.1 + scRNA-sequencing clusters. Colors correspond to clusters defined in Fig. . b Feature plots of region-defining eigengenes from hindbrain (HB; red), midbrain (MB; green), and optic tectum (OT; blue) overlaid on mpeg1.1 + UMAP, highlighting microglial clusters JM1 and JM4. Eigengenes were composed of single region marker genes with regional enrichment (log fold change >1.2, basemean >100, and adj. p < 0.05) from the bulk-sequencing analysis in Fig. and computed with the PercentageFeatureSet function in the Seurat R package. Color-coding for Eigengene expression: OT, low expression; light blue, high expression; dark blue. MB, low expression; light green, high expression; dark green. HB, low expression; pink, high expression; red. c Violin plots of regional eigengene distributions, related to b . Colors correspond with clusters shown in Figs. c, 4a. (Solid black line = median; dotted lines = 1st and 3rd quartiles). d Volcano plot of differentially expressed genes between clusters JM1 (OT-enriched; blue) and JM4 (hindbrain-enriched; red). Thresholds represented by dotted lines were set to adjusted p value < 10 −8 , log fold change >0.2. (MAST differential expression test with Bonferroni correction; See Supplemental S5). e Top GO terms from differentially expressed genes in d . (Metascape; hypergeometric test with Benjamini–Hochberg correction for multiple comparisons). f Representative images of cathepsin-cleaved Prosense680 colocalized with mpeg1.1- EGFP in indicated brain regions. Scale: 100 µm. Images are representative of the n = 3 replicates. g Quantification of total percent microglia containing cleaved Prosense680 in OT and HB. Unpaired t tests. Dots represent three individual fish, data are mean ± SD. * p < 0.0236. OT (Optic Tectum) and HB (Hindbrain). Two-tailed unpaired t test. h Representative images and 3D reconstructions of mpeg1.1- EGFP+ microglia with engulfed SV2 protein. Insets: close-up of reconstructions (arrowheads:SV2 content). Scale bar 5 µm. Images are representative of ameboid or ramified morphology. i Quantification of percent microglial volume containing SV2 in randomly selected microglia from OT and HB. Post hoc analyses show morphology assignment as ramified (red, sphericity <0.6) vs. ameboid (blue, sphericity >0.6. Mann–Whitney U test, * p < 0.0177, dots = mean value per fish from three microglia per fish. See also Fig. and Fig. , Supplementary Data .

Journal: Nature Communications

Article Title: In situ and transcriptomic identification of microglia in synapse-rich regions of the developing zebrafish brain

doi: 10.1038/s41467-021-26206-x

Figure Lengend Snippet: a Schematic of analysis pipeline overlaying brain-region-defining genes identified with bulk-sequencing onto mpeg1.1 + scRNA-sequencing clusters. Colors correspond to clusters defined in Fig. . b Feature plots of region-defining eigengenes from hindbrain (HB; red), midbrain (MB; green), and optic tectum (OT; blue) overlaid on mpeg1.1 + UMAP, highlighting microglial clusters JM1 and JM4. Eigengenes were composed of single region marker genes with regional enrichment (log fold change >1.2, basemean >100, and adj. p < 0.05) from the bulk-sequencing analysis in Fig. and computed with the PercentageFeatureSet function in the Seurat R package. Color-coding for Eigengene expression: OT, low expression; light blue, high expression; dark blue. MB, low expression; light green, high expression; dark green. HB, low expression; pink, high expression; red. c Violin plots of regional eigengene distributions, related to b . Colors correspond with clusters shown in Figs. c, 4a. (Solid black line = median; dotted lines = 1st and 3rd quartiles). d Volcano plot of differentially expressed genes between clusters JM1 (OT-enriched; blue) and JM4 (hindbrain-enriched; red). Thresholds represented by dotted lines were set to adjusted p value < 10 −8 , log fold change >0.2. (MAST differential expression test with Bonferroni correction; See Supplemental S5). e Top GO terms from differentially expressed genes in d . (Metascape; hypergeometric test with Benjamini–Hochberg correction for multiple comparisons). f Representative images of cathepsin-cleaved Prosense680 colocalized with mpeg1.1- EGFP in indicated brain regions. Scale: 100 µm. Images are representative of the n = 3 replicates. g Quantification of total percent microglia containing cleaved Prosense680 in OT and HB. Unpaired t tests. Dots represent three individual fish, data are mean ± SD. * p < 0.0236. OT (Optic Tectum) and HB (Hindbrain). Two-tailed unpaired t test. h Representative images and 3D reconstructions of mpeg1.1- EGFP+ microglia with engulfed SV2 protein. Insets: close-up of reconstructions (arrowheads:SV2 content). Scale bar 5 µm. Images are representative of ameboid or ramified morphology. i Quantification of percent microglial volume containing SV2 in randomly selected microglia from OT and HB. Post hoc analyses show morphology assignment as ramified (red, sphericity <0.6) vs. ameboid (blue, sphericity >0.6. Mann–Whitney U test, * p < 0.0177, dots = mean value per fish from three microglia per fish. See also Fig. and Fig. , Supplementary Data .

Article Snippet: To identify our putative OT-enriched cathepsin-rich cluster (JM1) in situ, we quantified functional cathepsin activity with the biomarker Prosense680, which becomes fluorescent after proteolytic cleavage by cathepsins with a preference for cathepsin B and L . Microglial cathepsin activity ( mpeg1.1 -EGFP + Prosense + ) was highly enriched in the OT, where it was mostly detected in ameboid microglia around neurogenic regions (Fig. ), closely matching the expression of ctsba by ISH (Fig. ).

Techniques: Sequencing, Marker, Expressing, Quantitative Proteomics, Two Tailed Test, MANN-WHITNEY